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JURNAL BIOLOGI INDONESIA

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Published by Lembaga Ilmu Pengetahuan Indonesia

ISSN : 08544425 EISSN : 2338834X DOI : -

Core Subject : Science,

Biochemistry, Genetics & Molecular Biology

Arjuna Subject : -

Articles 10 Documents

Search results for , issue " Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA" : 10 Documents clear

Konsumsi dan Efisiensi Pakan pada Kukang (Nycticebus coucang) di Penangkaran Wirdateti, Wirdateti; Puspitasari, Dewi; Diapari, Didid; Tjakradidjaja, Anita S
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTFeed Intake and Efficiency of Slow Loris (Nycticebus coucang) in Captivity. Study on feed intake and efficiency of captive slow loris (Nycticebus coucang) has been conducted at the Division of Zoolgy, Research Center for Biology â LIPI for five weeks. Three individuals were placed in three cages, with its sizes of 1.2 x 1.7 x 1.9 m (one cage) and 0.8 x 1.7 x 1.9 m (two cages). Feeds consisting of papaya, banana, coconut, passion fruit, guava, sweet corn, quail egg, and white bread, were given ad libitum. Average body weight of slow loris in the beginning of the study was 587 gram/head and at the end was 648.33 gram/head. Average of feed consumption was 317.26 gram/kgBW/day. Average of feed efficiency and protein efficiency ratio (PER) was 0.0270 and 0.0945, respectively.Key words: Feed, slow loris, Nycticebus coucang, captivity, feed efficiency, PER.

Variasi Genetik Monascus purpureus yang Diisolasi dari Beras Merah Cina Suharna, Nandang
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i3.3470

ABSTRACTGenetic Variation of Monascus purpureus Isolated from Chinese Red Rice. An attempt was carried out to detect genetic variation among six Monascus purpureus isolates by RAPD finger printing. The isolates studied were isolated from Chinese red rice (ang-kak) collected from Indonesian markets. Seven types of oligonucleotide 12-mer primers were used in this study. These primers were different from G+C ratio (42%, 50%, 68% and 75%) so as to know the best ratio for DNA amplication. Products of DNA amplication by seven primers were observed such as 158 banding patterns, comprise of 23 common fragments and 135 polimorfic fragments. The result showed that the six isolates were divided into two groups based on DNA banding patterns. The same grouping was also shown by phylogenetic analysis. This analysis indicated two cluster which were different from its genetic distance (49%) and two pairs of isolates were identical to each other. So far, this RAPD analysis proved that there was high genetic variation within the six isolat of M. purpureus. It was assumed that there was a tendency of species separation within those isolates. It is suggested that sequencing analysis on 18S rDNA should be carried out to ensure status of M.purpureus species used in ang-kak production.Key words: Monascus purpureus, variation, genetic, Chinese red-rice, RAPD

Pengaruh Introduksi Kompos Plus terhadap Produkasi Bobot Kering Daun Kumis Kucing (Orthosiphon aristatus) pada Tiga Macam Media Tanah Widawati, Sri; Suliasih, Suliasih; Syaifudin, Syaifudin
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTA green house experiment were conducted to study the application of âcompost plusâ on the growth of Orthosiphon aristatus. The experiment was designed in Complete Randomized Design with factorial and five replicates. The first factors were fertilizer application (compost plus, compost, and control). The second factors were 3 kinds of soil (i.e. soil from Cibinong, Ciomas, and Sukabumi). The result showed that compost plus application increased 113.90 gram/pot of dry weight of Orthosispon aristatus leaves compared with compost application and control in 3 kinds of soil.Key words : Compost plus (compost + microbes), Orthosispon aristatus

Pencirian Produksi Amilase oleh Saccaromyces cerevisiae W303A Rekombinan Thontowi, Ahmad; Puspaningsih, Ni Nyoman Tri; Hadi, Sofjan; Purkan, Purkan; Irawan, Bambang
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTCharacterization of Amylase Production by Saccharomyces cerevisiae W303A Recombinants. Cloning of amylase gene from Endomycopsis fibuligera ITB.R.cc.64 into S. cerevisiae W303a can effectively increase the yeast function to digest starch directly into ethanol. Production of amylase by S. cerevisiae W303a recombinants (I and P) were done by growing in yeast peptone starch (YPS) medium. The result showed that the recombinants could be produced of amylase by gave clear zone after staining by iodium vapor. The optimum condition of production of amylase by S. cerevisiae W303a recombinants were pH 7.0, 40?C temperature incubation, and gave maximum activity after 36 hours incubation. Amylase activity of I was higher than P recombinant for these condition respectively.Key words: Characterization, amylase, S. cerevisiae W303a

Efektivitas Spirulina sebagai Immunostilulan pada Ikan Patin Jambal (Pangasius djambal) Simanjuntak, Sorta Basar Ida; Wirawidjaja, Achmad Maad; Dana, Darnas; Supriyadi, Hambali
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTEffectivity of Immunostimulant Spirulina to Jambal Catfish (Pangasius djambal). Four kinds of diet with difference doses of Spirulina (0 gr.kg-1 diet; 2 gr.kg-1 diet; 4 gr.kg-1 diet and 6 gr.kg-1 diet) is experimented to jambal catfish (Pangasius djambal Bleeker). The aim of research to observed histology limphoid organs. This research is done during 28 days and challenged by intraperitoneal with Aeromonas hydrophila, one of pathogen bacteria on the 29th. The lymphoid organs of fish as taken every weeks to observed histology until the 42nd. The result showed that difference doses of Spirulina influence the histology of lymphoid organs jambal catfish. The dose of Spirulina 4 gr.kg-1 diet is effective to increase the immunity of jambal catfish bodies.Key Words : Spirulina, immunostimulant, lymphoid organ, Aeromonas hydrophila, jambal catfish

Biological Activity of Enzymatically Synthesized Polyphenol Glycoside on Microbial Growth Soeka, Yati Sudaryati; Widiasih, Lutfi Erlita; Sulistyo, Joko; Joko Sulistyo
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTBiological Activity of Enzymatically Synthesized Polyphenol Glycoside on Microbial Growth. We have studied an indigenous bacterial strain produced a glycosyl transfer enzyme (CGTase) yielding polyphenol glycosides from a substrate of starch and polyphenol-aglycone. We observed that the CGTase derived from culture filtrate of some microbial strains (Candida rugosa, Bacillus megaterium, B. coagulans and B. polymixa) could synthesize transfer products in the presence of appropriate polyphenol-aglycones as their acceptors. An inhibitory effects of enzymatically synthesized polyphenol glycosides against bacterial growth was furthermore examined. It was found that polyphenol-glycoside, as one of the transfer products, exhibited high antibacterial activity on the growth of Bacillus subtilis and Escherichia coli, no effect when on Bacillus cereus.Key words : Cyclodextrin glucano transferase (CGTase), enzymatic transglycosylation,polyphenol glycoside, antibacterial activity.

Metabolisme Benzonitril oleh Flavobacterium sp. NUB 1 Sulistinah, Nunik; Sunarko, Bambang; Thontowi, Ahmad
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTMetabolism of Benzonitriles by Flavobacterium sp. NUB 1. Flavobacterium sp. NUB 1 was isolated from industrial waste of PT. Petrokimia Gresik. The bacterium was able to utilize benzonitrile and acetonitrile and propionitril as the sole source of carbon and nitrogen. Growth on benzonitrile gave higher growth rate and biomass yield than growth on acetonitrile and propionitrile. When Flavovobacterium sp. NUB1 grew on benzonitril 15 mM , the doubling time is 9 hours 54 minutes and the specific growth rate (?) was 0,07 h-1. Whole cell of Flavobacterium sp. NUB 1 could hydrolyzed aromatic and aliphatic nitriles. The bacteria isolate has ability in metabolism of acetonitrile greater than benzonitrile. Activity of nitrile hydratase and amidase are more dominant than nitrilase in metabolism of benzonitrile.Key words: Biodegradation, benzonitril, Flavobacterium sp. NUB 1, nitrile-hydratase,amidase, nitrilase

Cendana (Santalum album ) dan Keanekaragaman Inang Sekundernya Di Daerah Pesisir Kolbano, Nusa Tenggara Timur Wawo, Albert H.; Abdulhadi, Rochadi
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTSandalwood (Santalum album ) and diversity of their host secondary in Kolbano Village, Lesser Sunda Island. The population of sandalwood (Santalum album ) declined every year because is not cultivated yet by the local people. Inhibiting factors are not possess the knowledge and skills on sandalwood cultivation especially in host plant of sandalwood comprehension. Forest fire and illegal logging lead to lose of flora diversity and deteriorate of sandalwood growth. Result of this research shows that population of sandalwood in Kolbano Village is very low are four plants in Kampong Fatu Un and seven plants in kampong Spaha. In kampong Fatu Un was found eight species plant that compose to unity formation with sandalwood and seven species of them are secondary host of sandalwood. In kampong Spaha was obtained nine species plant that compose to sandalwood formation and seven species of them are secondary host of sandalwood. The highest distribution frequency of plant in two mentioned kampong was got by leucaena (Leucaena glauca) plant with respectively value 51.28% (Fatu Un) and 53.57 % (Spaha), whereas diversity value belong to Simpson Index were 0.71 (Fatu Un) and 0.64 (Spaha).Key words: Sandalwood, host plant, Kolbano village, Lesser Sunda Island

Kemampuan Primer General CK4/CK2 Virus Infectious Bronchitis (IBV) untuk Mengaplifikasi Genom IBV Isolat Lapang Indonesia Dharmayanti, N.L P.I.; Asmara, W.; Artama, W. T.; Indriani, R.; Darminto, Darminto
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i3.3473

ABSTRACTCapability of general primer CK4/CK2 infectious bronchitis virus (IBV) to amplify of genomic IBV field isolates in Indonesia. Avian infectious bronchitis virus (IBV), the prototype of coronaviridae, is highly contangius, economically important pathogen of the chickens. Comparative sequence analysis of IBV S1 gene revealed regions that were conserved among serotypes. Two of conservesd region were used to develop degenarate general primer CK4/CK2 for amplifying IBV genomic RNA by RT-PCR. The aims of this study to demonstrated ability of general primer CK4/CK2 for amplified S1 gene IBV field isolates in Indonesia. The result of this is study suggest that only three IBV field isolates that can be amplified by primer CK4/CK2 that are I-14, I-37 and I-269.Key words: Capability, general primer CK4/CK2, infectious bronchitis virus, field isolates

Biodegradasi Alkil Benzena Sulfonat oleh Psedomonas cepacia Sudiana, I Made
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTAlkyl Benzene Sulfonate Biodegradation of Psedomonas cepacia. Alkyl Benzene Sulfonate (ABS), naturally slow biodegradable substances, and toxic to human, animal and microorganisms, is a focus of environmental studies. Microorganisms appeared to play important role on biodegradation of that substances in nature, and wastewater treatment procesess. S2 isolated from detergent contaminated soil was able to grow in media with ABS as the sole carbon source. ABS degradation took place under aerobic condition, at pH 4, temperature 30ÂºC with ?max of 0.0591-h, Ks = 3.25 mg/L, Vmax = 0.16 mg/L.hours-1, and Km = 14.52 mg/L. Analyses of 16s rDNA revealed that S2 is belonging to Pseudomonas cepacia.Key words: Alkyl Benzene Sulfononate (ABS), Pseudomonas cepacia, detergent

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